AFO may refer to:
- Absolute Force Online, a first-person shooting game.
- Advanced Force Operations
- Aero Force One, a fan club for the rock band Aerosmith
- AFO (Canada), L'assemblée de la Francophonie de l'Ontario, a cultural and political organization for Franco-Ontarians in the Canadian province of Ontario
- Air Force One, the air traffic control call sign of any United States Air Force aircraft carrying the President of the United States
- Air Force Ones (song), a 2002 song written and performed by St. Louis, Missouri hip hop artist Nelly
- Air Force 1 (shoe), a Nike, Inc. shoe design
- Alien Front Online, a Sega video game
- Animal feeding operation, as defined by the Environmental Protection Agency
- Anime Festival Orlando, a yearly anime convention in Orlando, Florida
- Ankle-foot orthosis, a brace for the ankle and foot
- Anti-Fascist Organisation, a provisional organization in Burma during World War II
- Aquatic Facilities Operator, a professional certification given by the National Recreation and Park Association
- Archiv für Orientforschung
- Arellano-Felix Organization, a drug trafficking cartel in Mexico, also known as "Tijuana Cartel"
- Arts Festival Oklahoma, a juried outdoor arts show in Oklahoma City every Labor Day weekend
- Association of Field Ornithologists, an ornithological organization, publishers of Journal of Field Ornithology
- Australian Field Ornithology, journal of Bird Observation & Conservation Australia
- Authorised Firearms Officer, a police officer in the United Kingdom who has received training and authorization to carry and use firearms
- Auto Fire Off, or Auto Fire Off ball, a ball in a fire extinguisher
- Odessa Photographers Association, in origin: Association of Fotographers in Odessa
- Apodo del Ingeniero en Electrónica Adolfo Juárez Morales
- Electronics Engineer nickname Adolfo Juárez Morales
- Afo, thought to be the world's oldest Clove Tree