The 2000 National League Division Series (NLDS), the opening round of the 2000 National League playoffs, began on Tuesday, October 3, and ended on Sunday, October 8, with the champions of the three NL divisions—along with a "wild card" team—participating in two best-of-five series. They were:
- (1) San Francisco Giants (Western Division champions, 97–65) vs. (4) New York Mets (Wild Card, 94–68): Mets win series, 3–1.
- (2) St. Louis Cardinals (Central Division champions, 95–67) vs. (3) Atlanta Braves (Eastern Division champions, 95–67): Cardinals win series, 3–0.
The higher seed (in parentheses) had the home field advantage (Games 1, 2 and 5 at home), which was determined by playing record. The Cardinals were awarded home field advantage rather than the Braves due to their 4–3 advantage in head-to-head play.
The Cardinals and Mets went on to meet in the NL Championship Series (NLCS). The Mets beat the Cardinals four games to one to advance to the 2000 World Series, where they would face the American League champion New York Yankees. With their division rival Atlanta Braves losing to the Cardinals, the Mets run to the World Series became much easier.
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