Technology
The DNA sequencing is done on a chip that contains many ZMWs. Inside each ZMW, a single active DNA polymerase with a single molecule of single stranded DNA template is immobilized to the bottom through which light can penetrate and create a visualization chamber that allows monitoring of the activity of the DNA polymerase at a single molecule level. The signal from a phospho-linked nucleotide incorporated by the DNA polymerase is detected as the DNA synthesis proceeds which results in the DNA sequencing in real time.
Read more about this topic: Single Molecule Real Time Sequencing
Famous quotes containing the word technology:
“The real accomplishment of modern science and technology consists in taking ordinary men, informing them narrowly and deeply and then, through appropriate organization, arranging to have their knowledge combined with that of other specialized but equally ordinary men. This dispenses with the need for genius. The resulting performance, though less inspiring, is far more predictable.”
—John Kenneth Galbraith (b. 1908)
“Our technology forces us to live mythically, but we continue to think fragmentarily, and on single, separate planes.”
—Marshall McLuhan (1911–1980)
“Primitive peoples tried to annul death by portraying the human body—we do it by finding substitutes for the human body. Technology instead of mysticism!”
—Max Frisch (1911–1991)