Single molecule real time sequencing (also known as SMRT) is a parallelized single molecule DNA sequencing by synthesis technology developed by Pacific Biosciences. Single molecule real time sequencing utilizes the zero-mode waveguide (ZMW), developed in the laboratories of Harold G. Craighead and Watt W. Webb at Cornell University. A single DNA polymerase enzyme is affixed at the bottom of a ZMW with a single molecule of DNA as a template. The ZMW is a structure that creates an illuminated observation volume that is small enough to observe only a single nucleotide of DNA (also known as a base) being incorporated by DNA polymerase. Each of the four DNA bases is attached to one of four different fluorescent dyes. When a nucleotide is incorporated by the DNA polymerase, the fluorescent tag is cleaved off and diffuses out of the observation area of the ZMW where its fluorescence is no longer observable. A detector detects the fluorescent signal of the nucleotide incorporation, and the base call is made according to the corresponding fluorescence of the dye. Sequence data generated from single molecule real time sequencing was first published in January 2009 in the journal Science.
Read more about Single Molecule Real Time Sequencing: Technology, Sequencing Performance, Application
Famous quotes containing the words single, molecule, real and/or time:
“When words fail us or, quite the opposite, when they rush from our mouths faster than we would like, we can console ourselves that if no single moment is going to define our relationship with a child, neither can a single lapse of good judgment or patience destroy it.”
—Cathy Rindner Tempelsman (20th century)
“We can come up with a working definition of life, which is what we did for the Viking mission to Mars. We said we could think in terms of a large molecule made up of carbon compounds that can replicate, or make copies of itself, and metabolize food and energy. So that’s the thought: macrocolecule, metabolism, replication.”
—Cyril Ponnamperuma (b. 1923)
“The real exertion in the case of an opera singer lies not so much in her singing as in her acting of a role, for nearly every modern opera makes great dramatic and physical demands.”
—Maria Jeritza (1887–1982)
“Young children...are often uninterested in conversation It is not that they don’t have ideas and feelings, or need to express them to others It is simply that as one eight-year-old boy once told me, “Talking is okay, but I don’t like to do it all the time the way grown-ups do; I guess you have to develop the habit.””
—Robert Coles (20th century)