Poot can refer to:
- Poot! (comics), a British adult comic
- Poot Carr, a fictional character on the HBO drama The Wire
- Marcel Poot, Belgian classical music composer
- Eduardo Avelino Magaña Poot (born 1984), Mexican archer
- Slang for flatulence
- A pet in My Teacher is an Alien
- Pootie Poot, George W. Bush's nickname for Vladimir Putin
- To aspirate, from the entomological term pooter (aspirator)