MBI may be:
- Management buy-in, the purchase of a large interest in a company by an investor group while retaining the existing management. (See Management buyout)
- Maslach Burnout Inventory, a scale for Burnout syndrome
- Master of Business Informatics
- Moody Bible Institute
- Machaneh Bonim in Israel, a tour of Israel by Habonim Dror
- Molecular breast imaging
- M/Bi (alternatively Bi/M), common shorthand abbreviation for bisexual male in a personal advertisement
- Minimum Background Investigation, security clearance