DNI may stand for:
- Director of National Intelligence, a U.S. cabinet-level official
- Director of Naval Intelligence (disambiguation)
- Direct neural interface, a brain-computer interface
- Do Not Intubate, an alternate term for "do not resuscitate"
- Documento Nacional de Identidad, the name of the national identity document in several Spanish-speaking countries.
- Dole Nutrition Institute founded by the Dole Food Company
- Direct Normal Irradiance, a measure of the solar irradiation striking a surface held normal to line of sight to the sun.
- Do Not Install, used in schematics.