Bambino is Italian for "little boy". The feminine is bambina. It can refer to:
- Il Bambino, name given in art to the image of the infant Jesus
- Bambino (grape), another name for the Italian wine grape Bombino bianco
- Bambino!, Japanese manga
- "Bambino" (Dalida song)
- "Bambino (Napoli Lullaby)", a song recorded by The Springfields
- "Bambina", a song included in Nue (Lara Fabian album)
- The Bambino, nickname of baseball legend Babe Ruth
- Bambino, Bud Spencer's character in the film They Call Me Trinity
- the same character in the sequel Trinity Is Still My Name
- Bambino (singer), Miguel Vargas Jiménez (1940–1999), Spanish singer
- Bambino (cat), a cross breed cat
- Bambini, 1951 Italian film
- The Fiat 500, known in Australia as "Bambino"
- Italian wine grape Cesanese Comune
- "Bambina," the nickname Debito has given to Felicita in the anime La storia della Arcana Famiglia