Advantages and Disadvantages of Yeast Display
Advantages of yeast display over other in vitro evolution methods include eukaryotic expression and processing, quality control mechanisms of the eukaryotic secretory pathway, minimal avidity effects, and quantitative library screening through fluorescent-activated cell sorting (FACS).
Disadvantages include smaller mutant library sizes compared to alternative methods and differential glycosylation in yeast compared to mammalian cells. These disadvantages have not limited the success of yeast display for a number of applications, including engineering the highest monovalent ligand-binding affinity reported to date for an engineered protein (Boder, E.T. 2000)
Alternative methods for protein evolution in vitro are phage display, ribosome display, bacterial display, and mRNA display.
Read more about this topic: Yeast Display
Famous quotes containing the words advantages, yeast and/or display:
“[T]here is no Part of the World where Servants have those Privileges and Advantages as in England: They have no where else such plentiful Diet, large Wages, or indulgent Liberty: There is no place wherein they labour less, and yet where they are so little respectful, more wasteful, more negligent, or where they so frequently change their Masters.”
—Richard Steele (1672–1729)
“Blues is to jazz what yeast is to bread—without it, it’s flat.”
—Carmen McRae (b. 1922)
“Lovers of painting and lovers of music are people who openly display their preference like a delectable ailment that isolates them and makes them proud.”
—Maurice Blanchot (b. 1907)