Function
Further work has established that the ydaO element is widespread and is associated with a diverse set of genes which it controls in a sequence and structure-dependent fashion. The natural ligand is present in cells grown a defined media and transposon-directed knockouts result in disregulation of associated genes.
A biochemical screen in Bacillus subtilis has shown that the ydaO motif specifically binds ATP and regulates the expression of genes in response to ATP. Thus demonstrating a role regulation of gene expression in response to energy balance in bacteria.
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