Culture Medium
| Venenivibrio stagnispumantis culture medium | |
|---|---|
| NaOH | 0.15 g |
| KCl | 0.50 g |
| MgCl2 • 6 H2O | 1.36 g |
| MgSO4 • 7 H2O | 7.00 g |
| Na2S2O3 • 5 H2O | 2.00 g |
| CaCl2 • 2 H2O | 0.40 g |
| NH4Cl | 0.20 g |
| K2HPO4 | 0.25 g |
| Trace minerals (100x) | 10.00 ml |
| MES | 1.95 g |
| Distilled water | make up to 1000.00 ml |
The pH of the medium is adjusted to 5.5, distributed into culture vessels and autoclaved under CO2. After the inoculation the initial gas phase is replaced with H2 / CO2 / O2 (79% / 15% / 6%) and pressurized to 170 kPa.
| Trace mineral solution (100x) | |
|---|---|
| Na2-EDTA • 2 H2O | 500.00 mg |
| CoCl2 • 6 H2O | 150.00 mg |
| MnCl2 • 4 H2O | 100.00 mg |
| FeSO4 • 7 H2O | 100.00 mg |
| ZnCl2 | 100.00 mg |
| AlCl3 • 6 H2O | 40.00 mg |
| Na2WO4 • 2 H2O | 30.00 mg |
| CuCl2 • 2 H2O | 20.00 mg |
| Ni2SO4 • 6 H2O | 20.00 mg |
| Na2SeO3 | 10.00 mg |
| H3BO3 | 10.00 mg |
| Na2MoO4 • 2 H2O | 10.00 mg |
| Distilled water | make up to 1000.00 ml |
The pH of the solution is adjusted to 3.0 with HCl.
Read more about this topic: Venenivibrio Stagnispumantis
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