Usui (written: 臼井 or 碓井) is a Japanese surname. Notable people with the surname include:
- Kaoru Usui (born 1916), photographer
- Usui Mikao (1865–1926), religious teacher, founder of Reiki
- Usui Sadamitsu, warrior
- Yoshimi Usui (1905–1987), author and critic
- Yoshito Usui (1958–2009), manga artist
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