Tunica may refer to:
- The Latin word for tunic, a type of clothing typical in the ancient world
- In animal anatomy, tunica refers to a membranous structure lining a cavity, or covering an organ such as a gland or a blood vessel; types include Tunica albuginea (disambiguation) and Tunica vasculosa (disambiguation)
- In Botany, tunica refers to a membranous structure such as the spermoderm, or any loose membrane not of epidermis, or the coat of a bulb or corm.
- Tunica, a flowering plant genus now included in Petrorhagia
- Tunica people, a Native American group in the Central Mississippi Valley
- Tunica (language), an isolate of the associated Tunica historic peoples in the Central Mississippi Valley
- Tunica, Louisiana
- Tunica, Mississippi
- Tunica Resorts, Mississippi (site of several casinos)
- Tunica County, Mississippi
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