Discovery of TmRNA and Early Work
tmRNA was first designated 10Sa RNA after a mixed “10S” electrophoretic fraction of Escherichia coli RNA was further resolved into tmRNA and the similarly-sized RNase P RNA (10Sb). The presence of pseudouridine in the mixed 10S RNA hinted that tmRNA has modified bases found also in tRNA. The similarity at the 3' end of tmRNA to the T stem-loop of tRNA was first recognized upon sequencing ssrA from Mycobacterium tuberculosis. Subsequent sequence comparison revealed the full tRNA-like domain (TLD) formed by the 5' and 3' ends of tmRNA, including the acceptor stem with elements like those in alanine tRNA that promote its aminoacylation by alanine-tRNA ligase. It also revealed differences from tRNA: the anticodon arm is missing in tmRNA, and the D arm region is a loop without base pairs.
Read more about this topic: Transfer-messenger RNA
Famous quotes containing the words discovery of, discovery, early and/or work:
“Your discovery of the contradiction caused me the greatest surprise and, I would almost say, consternation, since it has shaken the basis on which I intended to build my arithmetic.... It is all the more serious since, with the loss of my rule V, not only the foundations of my arithmetic, but also the sole possible foundations of arithmetic seem to vanish.”
—Gottlob Frege (1848–1925)
“We are all humiliated by the sudden discovery of a fact which has existed very comfortably and perhaps been staring at us in private while we have been making up our world entirely without it.”
—George Eliot [Mary Ann (or Marian)
“He had long before indulged most unfavourable sentiments of our fellow-subjects in America. For, as early as 1769,... he had said of them, “Sir, they are a race of convicts, and ought to be thankful for any thing we allow them short of hanging.””
—Samuel Johnson (1709–1784)
“You do not become a “dissident” just because you decide one day to take up this most unusual career. You are thrown into it by your personal sense of responsibility, combined with a complex set of external circumstances. You are cast out of the existing structures and placed in a position of conflict with them. It begins as an attempt to do your work well, and ends with being branded an enemy of society.”
—Václav Havel (b. 1936)