Cell Culture
Tetracycline is used in cell biology as a selective agent in cell culture systems. It is toxic to prokaryotic and eukaryotic cells and selects for cells harboring the bacterial tetr gene, which encodes a 399-amino-acid, membrane-associated protein. This protein actively exports tetracycline from the cell, rendering cells harboring this gene more resistant to the drug. The yellow crystalline powder can be dissolved in water (20 mg/ml) or ethanol (5 mg/ml), and is routinely used at 10 mg/l in cell culture. In cell culture at 37°C (99°F), it is stable for days, with a half-life of approximately 24 hours.
Read more about this topic: Tetracycline
Famous quotes containing the words cell and/or culture:
“I turn and turn in my cell like a fly that doesn’t know where to die.”
—Antonio Gramsci (1891–1937)
“Sanity consists in not being subdued by your means. Fancy prices are paid for position, and for the culture of talent, but to the grand interests, superficial success is of no account.”
—Ralph Waldo Emerson (1803–1882)