Cell Culture
Tetracycline is used in cell biology as a selective agent in cell culture systems. It is toxic to prokaryotic and eukaryotic cells and selects for cells harboring the bacterial tetr gene, which encodes a 399-amino-acid, membrane-associated protein. This protein actively exports tetracycline from the cell, rendering cells harboring this gene more resistant to the drug. The yellow crystalline powder can be dissolved in water (20 mg/ml) or ethanol (5 mg/ml), and is routinely used at 10 mg/l in cell culture. In cell culture at 37°C (99°F), it is stable for days, with a half-life of approximately 24 hours.
Read more about this topic: Tetracycline
Famous quotes containing the words cell and/or culture:
“What Mrs. Thatcher did for women was to demonstrate that if a woman had enough desire she could do what she wanted, do anything a man could do.... Mrs. Thatcher did not have one traditional feminine cell in her body.”
—Julie Burchill (b. 1960)
“As the traveler who has once been from home is wiser than he who has never left his own doorstep, so a knowledge of one other culture should sharpen our ability to scrutinize more steadily, to appreciate more lovingly, our own.”
—Margaret Mead (1901–1978)