PH Stability
Under basic (done by adding 0.12 M sodium carbonate until pH 11) and neutral conditions, steapsin activity decreased by 10% per hour. Under acidic conditions, it was discovered that only a small quantity of free hydrochloric acid (approximately 0.02M) was required to diminish the lipolytic activity of steapsin by 80% in the first hour. This suggests that steapsin is inactivated by acid and is present in the small intestines and not the stomach.
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