Spline may refer to:
- Spline (mechanical), a mating feature for rotating elements
- Spline (mathematics), a mathematical function used for interpolation or smoothing
- Smoothing spline, a method of smoothing using a spline function
- Flat spline, a device to draw curves
- Spline drive, a type of screw drive
- Spline (alien beings), fictional alien beings
- Spline cord, a type of thin rubber cord used to secure a window screen to its frame
- Spline, otherwise known as Star filler, is a type of plastic cable filler for CAT cable
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