The Process
The nucleus of a somatic cell is removed and kept, and the host's egg cell is kept and nucleus removed and discarded. Now we have a lone nucleus and an empty (or deprogrammed) egg cell. The lone nucleus is then fused with the 'deprogrammed' egg cell. After being inserted into the egg, the lone (somatic-cell) nucleus is reprogrammed by the host egg cell. The egg, now containing the somatic cell's nucleus, is stimulated with a shock and will begin to divide. After many mitotic divisions, this single cell forms a blastocyst (an early stage embryo with about 100 cells) with almost identical DNA to the original organism. The technique of transferring a nucleus from a somatic cell into an egg that produced Dolly was an extension of experiments that had been ongoing for over 40 years. In the simplest terms, the technique used to produce Dolly the sheep – somatic-cell nuclear transplantation cloning – involves removing the nucleus of an egg and replacing it with the diploid nucleus of a somatic cell.
Read more about this topic: Somatic-cell Nuclear Transfer
Famous quotes containing the word process:
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—Elizabeth Stuart Phelps (1844–1911)
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—James Conant (1893–1978)