SMRT may refer to:
- SMRT Corporation, a public transport operator in Singapore
- Seoul Metropolitan Rapid Transit Corporation, a company that operates Seoul Subway Lines 5 to 8
- Single Molecule Real Time Sequencing, a parallelized single molecule DNA sequencing by synthesis technology
- Silencing Mediator for Retinoid and Thyroid-hormone receptors, thyroid hormone receptors that are affected by nuclear receptor co-repressor 2
- St Mary Redcliffe and Temple School, a school located at Redcliffe, Bristol
- smrt, the Czech and Slovak word for "death"