RTX may refer to:
- Rooster Teeth Expo, the official fan convention for Rooster Teeth Productions
- RTX Red Rock, an action-adventure game for the PlayStation 2, which was developed and published by LucasArts
- Royal Trux, as an abbreviation for the name of the band
- Resiniferatoxin, a natural, high-potency ligand that activates the capsaicin (VR1, TRPV1) receptor
- The name of a firm involved in the defense industry featured in the film The Falcon and the Snowman
- A brand of external RAID storage by manufacturer WiebeTech. The acronym stands for "RAIDTech X" where X is a variable number of bays
- Real Time eXtensions for Windows (7/NT/2000/XP/XPe/Vista) made by IntervalZero
- RTX2010, a radiation-hardened microprocessor which has been used in numerous spacecraft
- RTX toxin, a type of toxin secreted by Gram-negative bacteria; RTX stands for repeats in toxin
- Rituximab, a monoclonal antibody used in the treatment of leukemia, lymphomas, transplant rejection and autoimmune disorders