Examples
- See the main article on list of restriction enzyme cutting sites.
Examples of restriction enzymes include:
| Enzyme | Source | Recognition Sequence | Cut |
|---|---|---|---|
| EcoRI | Escherichia coli | 5'GAATTC 3'CTTAAG | 5'---G AATTC---3' 3'---CTTAA G---5' |
| EcoRII | Escherichia coli | 5'CCWGG 3'GGWCC | 5'--- CCWGG---3' 3'---GGWCC ---5' |
| BamHI | Bacillus amyloliquefaciens | 5'GGATCC 3'CCTAGG | 5'---G GATCC---3' 3'---CCTAG G---5' |
| HindIII | Haemophilus influenzae | 5'AAGCTT 3'TTCGAA | 5'---A AGCTT---3' 3'---TTCGA A---5' |
| TaqI | Thermus aquaticus | 5'TCGA 3'AGCT | 5'---T CGA---3' 3'---AGC T---5' |
| NotI | Nocardia otitidis | 5'GCGGCCGC 3'CGCCGGCG | 5'---GC GGCCGC---3' 3'---CGCCGG CG---5' |
| HinfI | Haemophilus influenzae | 5'GANTCA 3'CTNAGT | 5'---G ANTC---3' 3'---CTNA G---5' |
| Sau3A | Staphylococcus aureus | 5'GATC 3'CTAG | 5'--- GATC---3' 3'---CTAG ---5' |
| PvuII* | Proteus vulgaris | 5'CAGCTG 3'GTCGAC | 5'---CAG CTG---3' 3'---GTC GAC---5' |
| SmaI* | Serratia marcescens | 5'CCCGGG 3'GGGCCC | 5'---CCC GGG---3' 3'---GGG CCC---5' |
| HaeIII* | Haemophilus aegyptius | 5'GGCC 3'CCGG | 5'---GG CC---3' 3'---CC GG---5' |
| HgaI | Haemophilus gallinarum | 5'GACGC 3'CTGCG | 5'---NN NN---3' 3'---NN NN---5' |
| AluI* | Arthrobacter luteus | 5'AGCT 3'TCGA | 5'---AG CT---3' 3'---TC GA---5' |
| EcoRV* | Escherichia coli | 5'GATATC 3'CTATAG | 5'---GAT ATC---3' 3'---CTA TAG---5' |
| EcoP15I | Escherichia coli | 5'CAGCAGN25NN 3'GTCGTCN25NN | 5'---CAGCAGN25NN ---3' 3'---GTCGTCN25 NN---5' |
| KpnI | Klebsiella pneumoniae | 5'GGTACC 3'CCATGG | 5'---GGTAC C---3' 3'---C CATGG---5' |
| PstI | Providencia stuartii | 5'CTGCAG 3'GACGTC | 5'---CTGCA G---3' 3'---G ACGTC---5' |
| SacI | Streptomyces achromogenes | 5'GAGCTC 3'CTCGAG | 5'---GAGCT C---3' 3'---C TCGAG---5' |
| SalI | Streptomyces albus | 5'GTCGAC 3'CAGCTG | 5'---G TCGAC---3' 3'---CAGCT G---5' |
| ScaI | Streptomyces caespitosus | 5'AGTACT 3'TCATGA | 5'---AGT ACT---3' 3'---TCA TGA---5' |
| SpeI | Sphaerotilus natans | 5'ACTAGT 3'TGATCA | 5'---A CTAGT---3' 3'---TGATC A---5' |
| SphI | Streptomyces phaeochromogenes | 5'GCATGC 3'CGTACG | 5'---GCATG C---3' 3'---C GTACG---5' |
| StuI | Streptomyces tubercidicus | 5'AGGCCT 3'TCCGGA | 5'---AGG CCT---3' 3'---TCC GGA---5' |
| XbaI | Xanthomonas badrii | 5'TCTAGA 3'AGATCT | 5'---T CTAGA---3' 3'---AGATC T---5' |
Key:
* = blunt ends
N = C or G or T or A
W = A or T
Read more about this topic: Restriction Enzyme
Famous quotes containing the word examples:
“Histories are more full of examples of the fidelity of dogs than of friends.”
—Alexander Pope (1688–1744)
“It is hardly to be believed how spiritual reflections when mixed with a little physics can hold people’s attention and give them a livelier idea of God than do the often ill-applied examples of his wrath.”
—G.C. (Georg Christoph)
“There are many examples of women that have excelled in learning, and even in war, but this is no reason we should bring ‘em all up to Latin and Greek or else military discipline, instead of needle-work and housewifry.”
—Bernard Mandeville (1670–1733)
Related Subjects
Related Phrases
Related Words