Components
It has one ampR gene (ampicillin resistance gene), and an N-terminal fragment of β-galactosidase (lac Z) gene of E. coli. The multiple cloning site (MCS) region is split into the lac Z gene (codons 6–7 of lac Z are replaced by MCS), where various restriction sites for many restriction endonucleases are present.
The ori site or replicon, rep is derived from pMB1 vector. pUC vector is small but has a high copy number. The high copy number of pUC plasmids is a result of the lack of the rop gene and a single point mutation in rep of pMB1. The lac Z gene codes for α-galactosidase. The recognition sites for HindIII, SphI, PstI, SalI, XbaI, BamHI, SmaI, KpnI, SacI and EcoRI restriction enzymes have been derived from the vector M13mp19.
Read more about this topic: PUC19
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“Hence, a generative grammar must be a system of rules that can iterate to generate an indefinitely large number of structures. This system of rules can be analyzed into the three major components of a generative grammar: the syntactic, phonological, and semantic components.”
—Noam Chomsky (b. 1928)