Primer Premier software combines design of primers for various PCR applications under one common platform. The software supports design of degenerate primers on alignments for amplifying related set of nucleotide sequences for detecting common traits among organisms and to determine heredity.
The software also designs tagged and nested primers for multiplex PCR reactions.
The Primer Premier software supports motif analysis and ORF search along with finding restriction cut sites on the nucleotide sequence.
The software has a built-in nucleotide-protein and protein-nucleotide sequence translator which can be used for designing degenerate primers for codons that code for multiple amino acid sequences. It is also understood that the primers designed by Primer Premier are effective and confirm to the design guidelines laid for designing primers for housekeeping genes with a high G+C%
The software also designs primers for flanking exons and introns in a gene, though there is no mention of it on the official Primer Premier page on the Premier Biosoft website.
The software calculates the hairpin structure stability and has been used to study its effect on PCR amplification
Read more about Primer Premier: Competitors
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—Anthony Munday (1553–1633)