Preliminary Messenger RNA - Processing

Processing

Eukaryotic pre-mRNA exists only briefly before it is fully processed into mRNA. Pre-mRNAs include two different types of segments, exons and introns. Exons are segments that are retained in the final mRNA, whereas introns are removed in a process called splicing, which is performed by the spliceosome (except for self-splicing introns).

Additional processing steps attach modifications to the 5' and 3' ends of Eukaryotic pre-mRNA. These include a 5' cap of 7-methylguanosine and a poly-A tail. In addition, eukaryotic pre-mRNAs have their introns spliced out by spliceosomes made up of small nuclear ribonucleoproteins.

When a pre-mRNA strand has been properly processed to an mRNA sequence, it is exported out of the nucleus and eventually translated into a protein – a process accomplished in conjunction with ribosomes.

In prokaryotes, the splicing is done either autocatalytically or through endolytic cleavage. Autocatalytic cleavages, in which no proteins are involved, are usually reserved for sections that code for rRNA, whereas endolytic cleavage corresponds to tRNA precursors.

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