Polymerase cycling assembly (or PCA, also known as Assembly PCR) is a method for the assembly of large DNA oligonucleotides from shorter fragments. The process uses the same technology as PCR, but takes advantage of DNA hybridization and annealing as well as DNA polymerase to amplify a complete sequence of DNA in a precise order based on the single stranded oligonucleotides used in the process. It thus allows for the production of synthetic genes and even entire synthetic genomes.
Read more about Polymerase Cycling Assembly: PCA Principles, Typical Reaction, Gibson Assembly, References
Famous quotes containing the words cycling and/or assembly:
“If all feeling for grace and beauty were not extinguished in the mass of mankind at the actual moment, such a method of locomotion as cycling could never have found acceptance; no man or woman with the slightest aesthetic sense could assume the ludicrous position necessary for it.”
—Ouida [Marie Louise De La Ramée] (1839–1908)
“There is a sacred horror about everything grand. It is easy to admire mediocrity and hills; but whatever is too lofty, a genius as well as a mountain, an assembly as well as a masterpiece, seen too near, is appalling.”
—Victor Hugo (1802–1885)