Nile Blue Staining
Nile blue is used for histological staining of biological preparations. It highlights the distinction between neutral lipids (triglycerides, cholesteryl esters, steroids) which are stained pink and acids (fatty acids, chromolipids, phospholipids) which are stained blue.
The Nile blue staining, according to Kleeberg, uses the following chemicals:
- Nile Blue A
- 1% acetic acid
- Glycerol or glycerol gelatin
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