Mylne is a surname, and may refer to:
- Alfred Mylne (1871–1951), Scottish yacht designer
- John Mylne (d.1621), Scottish master mason
- John Mylne (d.1657), "John Mylne of Perth", Scottish master mason
- John Mylne (1611–1667), "John Mylne junior", Scottish architect and master mason
- Louis George Mylne (1843–1941), Anglican Bishop of Bombay from 1876 to 1897
- Robert Mylne (1633–1710), Scottish architect and master mason
- Robert Mylne (1733–1811), Scottish architect and engineer
- Robert William Mylne (1817–1890), British architect and geologist
- Walter Myln (d. 1558), Scottish Protestant martyr
- William Mylne (1734–1790), Scottish architect and engineer
- William Chadwell Mylne (1781–1863), British engineer and architect