MTT Assays Significance
Tetrazolium dye reduction is dependent on NAD(P)H-dependent oxidoreductase enzymes largely in the cytosolic compartment of the cell. Therefore, reduction of MTT and other tetrazolium dyes increases with cellular metabolic activity due to elevated NAD(P)H flux. Resting cells such as thymocytes and splenocytes that are viable but metabolically quiet reduce very little MTT. In contrast, rapidly dividing cells exhibit high rates of MTT reduction. It is important to keep in mind that assay conditions can alter metabolic activity and thus tetrazolium dye reduction without affecting cell viability and that different tetarazolium dyes will give different results depending on whether they are reduced intracellularly (MTT, MTS) or extracellularly (WST-1).
Read more about this topic: MTT Assay
Famous quotes containing the words assays and/or significance:
“Your bait of falsehood takes this carp of truth,
And thus do we of wisdom and of reach,
With windlasses and with assays of bias,
By indirections find directions out.”
—William Shakespeare (1564–1616)
“Politics is not an end, but a means. It is not a product, but a process. It is the art of government. Like other values it has its counterfeits. So much emphasis has been placed upon the false that the significance of the true has been obscured and politics has come to convey the meaning of crafty and cunning selfishness, instead of candid and sincere service.”
—Calvin Coolidge (1872–1933)