Expression Patterns of MiR-199
Using TaqMan real-time quantitative PCR array methods, miRNA expression has been profiled. miR-199a, one of the most significantly overexpressed in invasive squamous cell carcinomas (ISCCs), was evaluated by transfecting cervical cancer cells (SiHa and ME-180) with anti-miR-199a oligonucleotides and the cell viability assessed. mirR-199a*, mir199a and mirR-199b were significantly overexpressed in ISCCs.
Comparative miRNA array led to the isolation of several Bone Morphogenic Protein 2 (BMP2)-responsive miRNAs. Among them, miR-199a* is of particular interest, because it was reported to be specifically expressed in the skeletal system.
Read more about this topic: Mir-199 Micro RNA Precursor
Famous quotes containing the words expression and/or patterns:
“Removed from its more restrictive sense, masturbation has become an expression for everything that has proved, for lack of human contact, to be void of meaning. We have communication problems, suffer from egocentrism and narcissism, are frustrated by information glut and loss of environment; we stagnate despite the rising GNP.”
—Günther Grass (b. 1927)
“For the man who should loose me is dead,
Fighting with the Duke in Flanders,
In a pattern called a war.
Christ! What are patterns for?”
—Amy Lowell (1874–1925)