Mayne may refer to:
- People
- Andrew Mayne, magician and filmmaker
- Brent Mayne (born 1968), American Baseball catcher
- Chris Mayne
- Clarice Mayne
- Cuthbert Mayne (1544–1577), English Roman Catholic priest and martyr
- Edgar Mayne (1882–1961), Australian cricketer
- Edith Mayne (born 1905)
- Edward Mayne (1756–1829)
- Ferdy Mayne (1916–1998)
- James O'Neil Mayne
- Jasper Mayne (1604–1672)
- John Mayne (1759–1836)
- Kenny Mayne (born 1959)
- Laurie Mayne (born 1942)
- Lennie Mayne (1928–1977)
- Lonnie Mayne
- Michael Mayne
- Mosley Mayne (1889–1955), British Indian Army officer
- Paddy Mayne (1915–1955), Northern Irish soldier and athlete
- Richard Mayne (1796–1868), first joint Commissioner of the Metropolitan Police
- Richard Charles Mayne (1835–1892), a Royal Navy officer prominent in the history of British Columbia
- Richard Mayne (administrator) (1926–2009), British international civil servant, writer
- Roger Mayne (born 1929), English photographer
- Seymour Mayne (born 1944), Canadian poet and literary translator
- Simon Mayne (1612–1661)
- Stephen Mayne (born 1969), Australian journalist
- Thom Mayne (born 1944)
- Thomas Mayne (disambiguation)
- Wiley Mayne
- William Mayne (1928–2010), British children's fiction author
- William Cyril Mayne (1877-1962), English clergyman and classical scholar
- Places
- Mayne Island, British Columbia
- Mayne Island Water Aerodrome
- Mayne Queen, ferry that serves the island
- Mayne Preceptory, a priory in Dorset, England
- Organisations
- Mayne Australian Football Club, Queensland
- Mayne Coaches, English
- Mayne Pharma, Australian drug company