Longhorn may refer to:
Animals:
- English Longhorn, a traditional long horned brown and white breed of cattle
- Texas longhorn (cattle), a breed of cattle
- Highland cattle, sometimes called Highland longhorn
- Longhorn beetle, a family of beetles with very long antennae
In the US state of Texas:
- Longhorn, Texas
- Longhorn Dam, on the Colorado River in Austin, Texas
- Texas Longhorns, sports teams and organizations at The University of Texas
- Mascot of J. Frank Dobie High School in Houston, Texas
- A candy of chocolate, pecans, and caramel made by Lammes Candies in Texas
- Longhorn Railway Company
Sport:
- Weinheim Longhorns, an American Football team from Weinheim, Germany
Technology:
- Longhorn or Longhorn Server, the code names of Windows Vista and Windows Server 2008 operating systems; see List of Microsoft codenames
Other uses:
- Longhorn (Transformers), one of several fictional characters in the various Transformers universes
- Longhorn cheddar, a type of colby cheese
- Longhorn Steakhouse, a chain of restaurants in the United States
- Jay's Longhorn Bar, a former music venue in Minneapolis commonly known as "The Longhorn"
- A fictional character on the animated television show Freakazoid!
- A piece of music used as the entrance theme for John Bradshaw Layfield, composed by Jim Johnston