DO
- DO – (s) Dominica (FIPS 10-4 country code) – Dominican Republic (ISO 3166 digram) – (i) Doctor of Osteopathic Medicine
- DOA – (i) Dead On Arrival – Dead or Alive
- DOB – (i) Date Of Birth
- DOD – (i) U.S. Department of Defense
- DODAF – (a) U.S. Department of Defense Architectural Framework
- DoDD – (i) U.S. Department of Defense Directive
- DoDEA – (i) (U.S.) Department of Defense Education Activity
- DOE – (i/a) U.S. Department of Energy
- DOHC – (i) Dual-OverHead-Cam engine
- DOI – (i) Digital Object Identifier – U.S. Department of the Interior
- DOM – (i) Deo optimo maximo (Latin, "to God, the best and greatest") – (a) Document Object Model – (s) Dominican Republic (ISO 3166 trigram)
- DOMS – (i) (U.S.) Director of Military Support
- DOP – (s) Dominican peso (ISO 4217 currency code)
- DORA – (a) Defence of the Realm Act
- DOS – (a) Denial of Service (DoS, cf. DDoS) – Density of State – Disk Operating System
- DOT – (i) Department of Transportation (U.S. federal or state) – Damage Over Time (common in World of Warcraft)
- DOTA – short-form for DEFENSE OF THE ANCIENTS a popular online epic game
- DotD – (i) Deal of the Day (marketing gimmick)
- DOTMLPF – (i) Doctrine, Organization, Training, Materiel, Leadership, Personnel, and Facilities (mnemonic)
- DOW – (i) Died of Wounds
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