Imaging Techniques
Recent developments in MALDI methods have enabled direct detection of lipids in-situ. Abundant lipid-related ions are produced from the direct analysis of thin tissue slices when sequential spectra are acquired across a tissue surface that has been coated with a MALDI matrix. Collisional activation of the molecular ions can be used to determine the lipid family and often structurally define the molecular species. This technique enables detection of phospholipids, sphingolipids and glycerolipids in tissues such as heart, kidney and brain. Furthermore distribution of many different lipid molecular species often define anatomical regions within these tissues.
Read more about this topic: Lipidomics
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