Use in Stem Cell Culture
Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog.
LIF is not required for culture of human embryonic stem cells.
Read more about this topic: Leukemia Inhibitory Factor
Famous quotes containing the words stem, cell and/or culture:
“The lotus’ stem is as long as the depth of water,
So men’s height is just as great as their inner strength.”
—Tiruvalluvar (c. 5th century A.D.)
“Let man consider what he is in comparison with all existence; let him regard himself as lost in this remote corner of nature; and from the little cell in which he finds himself lodged, I mean the universe, let him estimate at their true value the earth, kingdoms, cities, and himself. What is a man in the infinite?”
—Blaise Pascal (1623–1662)
“The local is a shabby thing. There’s nothing worse than bringing us back down to our own little corner, our own territory, the radiant promiscuity of the face to face. A culture which has taken the risk of the universal, must perish by the universal.”
—Jean Baudrillard (b. 1929)