Research
Because the 5' → 3' exonuclease activity of DNA polymerase I from E. coli makes it unsuitable for many applications, the Klenow fragment, which lacks this activity, can be very useful in research. The Klenow fragment is extremely useful for research-based tasks such as:
- Synthesis of double-stranded DNA from single-stranded templates
- Filling in (meaning removal of overhangs to create blunt ends) recessed 3' ends of DNA fragments
- Digesting away protruding 3' overhangs
- Preparation of radioactive DNA probes
The Klenow fragment was also the original enzyme used for greatly amplifying segments of DNA in the polymerase chain reaction (PCR) process, before being replaced by thermostable enzymes such as Taq polymerase.
Read more about this topic: Klenow Fragment
Famous quotes containing the word research:
“Feeling that you have to be the perfect parent places a tremendous and completely unnecessary burden on you. If we’ve learned anything from the past half-century’s research on child development, it’s that children are remarkably resilient. You can make lots of mistakes and still wind up with great kids.”
—Lawrence Kutner (20th century)
“If politics is the art of the possible, research is surely the art of the soluble. Both are immensely practical-minded affairs.”
—Peter B. Medawar (1915–1987)
“Our science has become terrible, our research dangerous, our findings deadly. We physicists have to make peace with reality. Reality is not as strong as we are. We will ruin reality.”
—Friedrich Dürrenmatt (1921–1990)