John Kline is the name of:
- John Kline (Harlem Globetrotter) (fl. 1950s), retired Harlem Globetrotter (1953–1959) who founded the Black Legends of Professional Basketball in 1996
- John Kline (politician) (born 1947), American congressman from Minnesota
- John Robert Kline (1891–1955), American mathematician
- Johnny Klein (1918–1997), American musician