Typical Technique
A sample is introduced, either manually or with an autosampler, into a sample loop of known volume. A buffered aqueous solution known as the mobile phase carries the sample from the loop onto a column that contains some form of stationary phase material. This is typically a resin or gel matrix consisting of agarose or cellulose beads with covalently bonded charged functional groups. The target analytes (anions or cations) are retained on the stationary phase but can be eluted by increasing the concentration of a similarly charged species that will displace the analyte ions from the stationary phase. For example, in cation exchange chromatography, the positively charged analyte could be displaced by the addition of positively charged sodium ions. The analytes of interest must then be detected by some means, typically by conductivity or UV/Visible light absorbance.
In order to control an IC system, a chromatography data system (CDS) is usually needed. In addition to IC systems, some of these CDSs can also control gas chromatography (GC) and HPLC
Read more about this topic: Ion Chromatography
Famous quotes containing the words typical and/or technique:
“The books may say that nine-month-olds crawl, say their first words, and are afraid of strangers. Your exuberantly concrete and special nine-month-old hasn’t read them. She may be walking already, not saying a word and smiling gleefully at every stranger she sees. . . . You can support her best by helping her learn what she’s trying to learn, not what the books say a typical child ought to be learning.”
—Amy Laura Dombro (20th century)
“I cannot think that espionage can be recommended as a technique for building an impressive civilisation. It’s a lout’s game.”
—Rebecca West (1892–1983)