Infectious Hematopoietic Necrosis Virus - Diagnosis

Diagnosis

Clinical signs and history of previous outbreaks may be suggestive of IHN.

Staphylococcal agglutination, virus neutralisation (VN), indirect fluorescent antibody testing, ELISA, PCR and DNA probe technology are all techniques that can be used to confirm diagnosis. The gold standard is virus neutralisation. Alternatively, the identification of degeneration and necrosis of granular cells in the lamina propria, stratum compactum and stratum granulosum of the gastrointestinal tract on histopathology can be used to diagnose infection.