Illumina may refer to:
- Illumina (Alisha's Attic album), an album by Alisha's Attic
- Illumina (Two Steps from Hell album), an album by Two Steps from Hell
- the sword Illumina (originally Lightbringer), one of the Final Fantasy weapons
- Illumina (company), a biotechnology company based in San Diego, California, United States
- Illuminas, a market research consultancy