Uses
The HILIC mode of separation is used extensively for separation of some biomolecules, organic and some inorganic molecules by differences in polarity. Its utility has increased due to the simplified sample preparation for biological samples, when analyzing for metabolites, since the metabolic process generally results in the addition of polar groups to enhance elimination from the cellular tissue. For the detection of polar compounds with the use of electrospray-ionization mass spectrometry as a chromatographic detector, HILIC can offer a tenfold increase in sensitivity over reversed-phase chromatography because the organic solvent is much more volatile.
Read more about this topic: Hydrophilic Interaction Chromatography