Golgi's method is a nervous tissue staining technique discovered by Italian physician and scientist Camillo Golgi (1843–1926) in 1873. It was initially named the black reaction (la reazione nera) by Golgi, but it became better known as the Golgi stain or later, Golgi method.
Golgi' staining was famously used by Spanish neuroanatomist Santiago Ramón y Cajal (1852–1934) to discover a number of novel facts about the organization of the nervous system, inspiring the birth of the neuron doctrine. Ultimately, Ramon y Cajal improved the technique by using a method he termed "double impregnation." Ramon y Cajal's staining technique, still in use, is called Cajal's Stain.
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