Activity
At pH 7, glucose exists in solution in cyclic hemiacetal form as 63.6% β-D-glucopyranose and 36.4% α-D-glucopyranose, the proportion of linear and furanose form being negligible. The glucose oxidase binds specifically to β-D-glucopyranose and does not act on α-D-glucose. It is able to oxidise all of the glucose in solution because the equilibrium between the α and β anomers is driven towards the β side as it is consumed in the reaction.
Glucose oxidase catalyzes the oxidation of β-D-glucose into D-glucono-1,5-lactone, which then hydrolyzes to gluconic acid.
In order to work as a catalyst, GOx requires a cofactor, flavin adenine dinucleotide (FAD). FAD is a common component in biological oxidation-reduction (redox reactions). Redox reactions involve a gain or loss of electrons from a molecule. In the GOx-catalyzed redox reaction, FAD works as the initial electron acceptor and is reduced to FADH2. Then FADH2 is oxidized by the final electron acceptor, molecular oxygen (O2), which can do so because it has a higher reduction potential. O2 is then reduced to hydrogen peroxide (H2O2).
Read more about this topic: Glucose Oxidase
Famous quotes containing the word activity:
“Life is a series of diminishments. Each cessation of an activity either from choice or some other variety of infirmity is a death, a putting to final rest. Each loss, of friend or precious enemy, can be equated with the closing off of a room containing blocks of nerves ... and soon after the closing off the nerves atrophy and that part of oneself, in essence, drops away. The self is lightened, is held on earth by a gram less of mass and will.”
—Coleman Dowell (1925–1985)
“Every time a child organizes and completes a chore, spends some time alone without feeling lonely, loses herself in play for an hour, or refuses to go along with her peers in some activity she feels is wrong, she will be building meaning and a sense of worth for herself and harmony in her family.”
—Barbara Coloroso (20th century)
“The Good of man is the active exercise of his soul’s faculties in conformity with excellence or virtue.... Moreover this activity must occupy a complete lifetime; for one swallow does not make spring, nor does one fine day; and similarly one day or a brief period of happiness does not make a man supremely blessed and happy.”
—Aristotle (384–322 B.C.)