Generation
Giemsa's solution is a mixture of methylene blue, eosin, and azure B. The stain is usually prepared from commercially available Giemsa powder.
A thin film of the specimen on a microscope slide is fixed in pure methanol for 30 seconds, by immersing it or by putting a few drops of methanol on the slide. The slide is immersed in a freshly prepared 5% Giemsa stain solution for 20–30 minutes (in emergencies 5–10 minutes in 10% solution can be used), then flushed with tap water and left to dry.
Read more about this topic: Giemsa Stain
Famous quotes containing the word generation:
“... if women once learn to be something themselves, that the only way to teach is to be fine and shining examples, we will have in one generation the most remarkable and glorious children.”
—Brenda Ueland (1891–1985)
“We were that generation called “silent,” but we were silent neither, as some thought, because we shared the period’s official optimism nor, as others thought, because we feared its official repression. We were silent because the exhilaration of social action seemed to many of us just one more way of escaping the personal, of masking for a while that dread of the meaningless which was man’s fate.”
—Joan Didion (b. 1935)
“My generation was ... haunted by Dr. Freud’s psychoanalysis, which ... had become the latest rage among the intelligentsia. I feel sorry for the modern mothers who have not one but a whole swarm of famous psychiatrists to confuse their thinking and spoil their fun by reminding them constantly that love is a dangerous business.”
—Agnes E. Meyer (1887–1970)