Generation
Giemsa's solution is a mixture of methylene blue, eosin, and azure B. The stain is usually prepared from commercially available Giemsa powder.
A thin film of the specimen on a microscope slide is fixed in pure methanol for 30 seconds, by immersing it or by putting a few drops of methanol on the slide. The slide is immersed in a freshly prepared 5% Giemsa stain solution for 20–30 minutes (in emergencies 5–10 minutes in 10% solution can be used), then flushed with tap water and left to dry.
Read more about this topic: Giemsa Stain
Famous quotes containing the word generation:
“A generation which has passed through the shop has absorbed standards and ambitions which are not of those of spaciousness, and cannot get away from them. Everything with them is done as though for sale, and they naturally have in view the greatest possible benefit, profit and that end of the stuff that will make the best show.”
—Alexander Herzen (1812–1870)
“In colonial America, the father was the primary parent. . . . Over the past two hundred years, each generation of fathers has had less authority than the last. . . . Masculinity ceased to be defined in terms of domestic involvement, skills at fathering and husbanding, but began to be defined in terms of making money. Men had to leave home to work. They stopped doing all the things they used to do.”
—Frank Pittman (20th century)
“My generation had Doris Day as a role model, then Gloria Steinem—then Princess Diana. We are the most confused generation.”
—Erica Jong (b. 1942)