Science
Many food additives absorb radiation in the ultraviolet and / or visible region of the spectrum. This absorbance can be used to determine the concentration of an additive in a sample using external calibration. However, additives may occur together and the absorbance by one could interfere with the absorbance of another. A prior separation stage is necessary and the additives are first separated by high liquid chromatography (HPLC) and then determined on-line using a UV and/or visible detector.
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