Common Fluorescent Probes
The fluorescent species used in FCS is typically a biomolecule of interest that has been tagged with a fluorophore (using immunohistochemistry for instance), or is a naked fluorophore that is used to probe some environment of interest (e.g. the cytoskeleton of a cell). The following table gives diffusion coefficients of some common fluorophores in water at room temperature, and their excitation wavelengths.
| Fluorescent dye | (×10−10 m2 s−1) | Excitation wavelength (nm) | Reference | |
|---|---|---|---|---|
| Rhodamine 6G | 2.8, 3.0, 4.14 ± 0.05 @ 25.00 °C | 514 | , | |
| Rhodamine 110 | 2.7 | 488 | ||
| Tetramethyl rhodamine | 2.6 | 543 | ||
| Cy3 | 2.8 | 543 | ||
| Cy5 | 2.5, 3.7 ± 0.15 @ 25.00 °C | 633 | , | |
| carboxyfluorescein | 3.2 | 488 | ||
| Alexa-488 | 1.96,4.35 @ 22.5±0.5 °C | 488 | ||
| Atto655-maleimide | 4.07 ± 0.1 @ 25.00 °C | 663 | ||
| Atto655-carboxylicacid | 4.26 ± 0.08 @ 25.00 °C | 663 | ||
| 2′, 7′-difluorofluorescein (Oregon Green488) | 4.11 ± 0.06 @ 25.00 °C | 498 |
Read more about this topic: Fluorescence Correlation Spectroscopy
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