Enzyme Multiplied Immunoassay Technique - Technique

Technique

  1. Combine a sample suspected of containing the drug of interest with a solution containing a known concentration of antibody and the enzyme substrate.
  2. After a short period (usually less than a minute) to allow binding, add a known concentration of the conjugate.
  3. Measure the rate of appearance of color or fluorescence.
  4. Determine the drug concentration by comparing the observed rate to the rates produced by known concentrations of the drug.

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