Technique
- Combine a sample suspected of containing the drug of interest with a solution containing a known concentration of antibody and the enzyme substrate.
- After a short period (usually less than a minute) to allow binding, add a known concentration of the conjugate.
- Measure the rate of appearance of color or fluorescence.
- Determine the drug concentration by comparing the observed rate to the rates produced by known concentrations of the drug.
Read more about this topic: Enzyme Multiplied Immunoassay Technique
Famous quotes containing the word technique:
“A successful social technique consists perhaps in finding unobjectionable means for individual self-assertion.”
—Eric Hoffer (1902–1983)
“The mere mechanical technique of acting can be taught, but the spirit that is to give life to lifeless forms must be born in a man. No dramatic college can teach its pupils to think or to feel. It is Nature who makes our artists for us, though it may be Art who taught them their right mode of expression.”
—Oscar Wilde (1854–1900)
“The audience is the most revered member of the theater. Without an audience there is no theater. Every technique learned by the actor, every curtain, every flat on the stage, every careful analysis by the director, every coordinated scene, is for the enjoyment of the audience. They are our guests, our evaluators, and the last spoke in the wheel which can then begin to roll. They make the performance meaningful.”
—Viola Spolin (b. 1911)