Technique
- Combine a sample suspected of containing the drug of interest with a solution containing a known concentration of antibody and the enzyme substrate.
- After a short period (usually less than a minute) to allow binding, add a known concentration of the conjugate.
- Measure the rate of appearance of color or fluorescence.
- Determine the drug concentration by comparing the observed rate to the rates produced by known concentrations of the drug.
Read more about this topic: Enzyme Multiplied Immunoassay Technique
Famous quotes containing the word technique:
“Technique is the test of sincerity. If a thing isn’t worth getting the technique to say, it is of inferior value.”
—Ezra Pound (1885–1972)
“The mere mechanical technique of acting can be taught, but the spirit that is to give life to lifeless forms must be born in a man. No dramatic college can teach its pupils to think or to feel. It is Nature who makes our artists for us, though it may be Art who taught them their right mode of expression.”
—Oscar Wilde (1854–1900)
“I cannot think that espionage can be recommended as a technique for building an impressive civilisation. It’s a lout’s game.”
—Rebecca West (1892–1983)