Reproduction
Temperature has been shown to change the length of time it takes them to reach reproductive maturity and body size. This species is also known to give birth to live young instead of laying a clutch of eggs. The breeding season for Gambusia holbrooki is between midspring and midautumn, with the peak breeding time being around summer. Females can have up to nine broods per mating season, with the average size ranging from five to 100. The variability of the average brood size is due to many variables, including temperature, age, and available nutrients. Higher temperatures have been shown to increase the fecundity of this species. The gestation period for this species is between 22 and 25 days.
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