DOB or Dob may refer to:
- Dob, Domžale, village in Slovenia
- Mounir Dob (born 1974), Algerian football referee
- Date of birth or birthday (often DoB)
- 2,5-dimethoxy-4-bromoamphetamine, Bromo-DMA, a psychedelic drug
- Meta-DOB, related substance
- Methyl-DOB, related substance
- HLA-DOB, human gene
- Daughters of Bilitis, an international lesbian and feminist organization
- Division of Banks, an Australian Electoral Division in New South Wales
- Dust of Basement, an electronic music band originally from Berlin
- New York City Transit Department of Buses, known as MTA New York City Transit buses
- Deutsche Oper Berlin
- In astronomy, slang for a Dobsonian telescope
- Dykes on Bikes, international network of motorcycle clubs
- NATO Dispersed Operating Bases (DOBs)
- Daily Oil Bulletin, a Canadian petroleum industry publication published every week day
- A slang or colloquial term for a kick in Australian Rules Football
- Daniel O'Brien (comedian), writer for Cracked.com
- Dave O'Brien (tactical genius)
- Dobo Airport, Indonesia (IATA code DOB)