Choice of Locus
A desirable locus for DNA barcoding should be standardized (so that large databases of sequences for that locus can be developed), present in most of the taxa of interest and sequencable without species-specific PCR primers, short enough to be easily sequenced with current technology, and provide a large variation between species yet a relatively small amount of variation within a species.
Although several loci have been suggested, a common set of choices are:
- For animals and many other eukaryotes, the mitochondrial CO1 gene
- For land plants, the concatenation of the rbcL and matK chloroplast genes
Read more about this topic: DNA Barcoding
Famous quotes containing the words choice of, choice and/or locus:
“Come and take choice of all my library,
And so beguile thy sorrow.”
—William Shakespeare (1564–1616)
“Live a thousand years,
I shall not find myself so apt to die.
No place will please me so, no mean of death,
As here by Caesar, and by you cut off,
The choice and master spirits of this age.”
—William Shakespeare (1564–1616)
“Seeing the locus of joy as the gate
of a city, or as a lych-gate ...”
—Denise Levertov (b. 1923)