Applications
The technique is used to:
- Homogenize cells and tissues
- Release intact organelles
- Prepare cell membranes
- Release labile biochemicals
- Produce uniform and repeatable homogenates without subjecting the sample to extreme chemical or physical stress.
According to manufacturers of nitrogen decompression devices, the method is particularly well suited for treating mammalian and other membrane bound cells. It has also been used successfully for treating plant cells, for releasing virus from fertilized eggs and for treating fragile bacteria. It is not recommended for untreated bacterial cells. Yeast, fungus, spores and other materials with tough cell walls do not respond well to this method.
Read more about this topic: Cell Disruption By Nitrogen Decompression